TCP1 alpha/CCTA anticorps (C-Term)

N° du produit ABIN215497

Cet anticorps Rat Monoclonal détecte spécifiquement TCP1 alpha/CCTA dans WB, IP et ICC. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Hamster, Singe, Cobaye, Porc et Drosophila melanogaster.

Aperçu rapide pour TCP1 alpha/CCTA anticorps (C-Term) (ABIN215497)

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Hamster, Singe, Cobaye, Porc, Drosophila melanogaster

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TCP1 alpha/CCTA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-TCP1 alpha/CCTA anticorps

Épitope: C-Term

Specificité: This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.

Purification: Protein G purified

Immunogène: A purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ICC (5 μg/mL), IP (12.5 μg/mL), WB

Usage: Western Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL 5 μg/mL. Immunocytochemistry: 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, in 50 % glycerol.

Agent conservateur: PMSF

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur TCP1 alpha/CCTA

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Autre désignation: TCP1

Sujet: Name/Gene ID: TCP1

Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1

ID gène: 6950

UniProt: P17987