Radioprotective 105 anticorps

N° du produit ABIN2191770

Cet anticorps anti- est un anticorps Rat Monoclonal détectant dans Act, Func et FACS. Adapté pour Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Radioprotective 105 anticorps (ABIN2191770)

Antigène: Radioprotective 105

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Application: Activation (Act), Functional Studies (Func), Flow Cytometry (FACS)

Clone: RP-14

Détail du produit

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

Nagai, Shimazu, Ogata, Akashi, Sudo, Yamasaki, Hayashi, Iwakura, Kimoto, Miyake: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide." dans: Blood, Vol. 99, Issue 5, pp. 1699-705, (2002) (PubMed).

Détail du antigène

Antigène: Radioprotective 105

Sujet: Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-riche repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely + restricted to immune cells including CD19 B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.