F4/80 anticorps

N° du produit ABIN2191819

Cet anticorps Rat Monoclonal détecte spécifiquement F4/80 dans FACS, WB et IHC (p). Il présente une réactivité avec des échantillons de Souris. Il a été cité dans 3+ publications.

Aperçu rapide pour F4/80 anticorps (ABIN2191819)

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BM8

Détail du produit anti-F4/80 anticorps

Réactivité croisée (Details): Cross reactivity: Mouse granulocytes : No, Mouse mast cells : No, Mouse platelets : No, Mouse lymphocytes : No, Mouse fibroblasts : No, Mouse endothelial cells : No

Stérilité: 0.2 μm filtered

Isotype: IgG2a

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse macrophages control Negative Mouse fibroblasts or granulocytes control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-F4/80 (ABIN2191819)

Schaller, Macfarlane, Rupec, Gordon, McKnight, Pfeffer: "Inactivation of the F4/80 glycoprotein in the mouse germ line." dans: Molecular and cellular biology, Vol. 22, Issue 22, pp. 8035-43, (2002) (PubMed).

Mackler, Iezza, Akin, McMillan, Yellon: "Macrophage trafficking in the uterus and cervix precedes parturition in the mouse." dans: Biology of reproduction, Vol. 61, Issue 4, pp. 879-83, (1999) (PubMed).

Leenen, de Bruijn, Voerman, Campbell, van Ewijk: "Markers of mouse macrophage development detected by monoclonal antibodies." dans: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, (1994) (PubMed).

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Sujet: The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 Molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Immunogen BALB/c macrophages obtained from 14-day-old bone marrow cell cultures