Beta3 Integrin Subunit anticorps

N° du produit ABIN2191904

L’anticorps Souris Monoclonal anti- a été validé pour Func, IHC (fro), IP et IA. Il convient pour détecter dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour Beta3 Integrin Subunit anticorps (ABIN2191904)

Antigène: Beta3 Integrin Subunit

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunoassay (IA)

Clone: BV4

Détail du produit

Réactivité croisée (Details): Cross reactivity: Bovine : Yes

Stérilité: 0.2 μm filtered

Isotype: IgG1

Information d'application

Indications d'application: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Endometrium control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Référence

Neto, Pantaleão, de Sá, Landman: "Alpha-v-beta3 integrin expression in melanocytic nevi and cutaneous melanoma." dans: Journal of cutaneous pathology, Vol. 34, Issue 11, pp. 851-6, (2007) (PubMed).

Soldi, Mitola, Strasly, Defilippi, Tarone, Bussolino: "Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2." dans: The EMBO journal, Vol. 18, Issue 4, pp. 882-92, (1999) (PubMed).

Détail du antigène

Antigène: Beta3 Integrin Subunit

Sujet: The monoclonal antibody BV4 recognizes human beta3 integrin subunit present in Platelet glycoprotein GPIIb-IIIa (integrin alphaIIb/beta3, CD41/CD61) and in the vitronectin receptor (integrin alphaV/beta3, CD51/CD61). Intergins are a family of heterodimeric membrane glycoproteins expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. As adhesion molecules they play an important role in numerous biological processes such as platelet aggregation, inflammation, immune function, wound healing, tumour metastasis and tissue migration during embryogenesis. In addition integrins are involved in signaling pathways, transmitting signals both into an out from cells. All integrins consist of two non- covalently associated subunits, alpha and beta. At least 12 different alpha subunits and 8 beta subunits have been identified. The beta subunits all contain 56 conserved cysteines (except beta4 which has 48) which are arranged in four repeating units. The beta3 subunit is a 93 kDa protein that contains a large loop in the N-terminus stabilized by intrachain disulphide bonding with the first cysteine-rich repeat. Platelet glycoprotein GPIIb-IIIa is expressed on platelets and megakaryoblasts. It is constitutively expressed and becomes activated on triggered platelets. Platelet glycoprotein GPIIb-IIIa binds to fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Next to this it is also a receptor for several soluble adhesive proteins.Vitronectin receptor is expressed on endothelial cells, some B cells, monocytes/macrophages, platelets and tumour cells. Vitronectin receptor binds next to vitronectin to fibrinogen, vWF, thrombospondin, fibronectin, osteopontin and collagen. Defects in human beta3 integrin are a cause of Glanzmann thrombasthenia, which is an autosomal recessive disorder characterized by mucocutaneous bleeding and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. Aliases Platelet membrane glycoprotein IIIa, GPIIIa