Cité dans 3+ publications.
Cet anticorps anti-Lipid A est un anticorps Monoclonal produit chez le Souris, détectant Lipid A dans WB. Convient pour Chemical.
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Restrictions
For Research Use only
Buffer
1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.
Date de péremption
12 months
Chou, Chuang, Eng, Chen, Wang, Chen, Lin: "Endotoxin and CD14 in the progression of biliary atresia." dans: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).
Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed." dans: Immunobiology, Vol. 189, Issue 5, pp. 457-71, (1994) (PubMed).
Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A." dans: Infection and immunity, Vol. 60, Issue 6, pp. 2201-10, (1992) (PubMed).
Antigène
Lipid A
Classe de substances
Chemical
Sujet
The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.