FHL2 anticorps

N° du produit ABIN2192004

Cet anticorps anti-FHL2 est un anticorps Souris Monoclonal détectant FHL2 dans WB, IF, IHC (p) et IHC (fro). Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour FHL2 anticorps (ABIN2192004)

Antigène: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FHL2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F4B2-B11

Détail du produit anti-FHL2 anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-FHL2 (ABIN2192004)

Martin, Schneider, Janetzky, Waibler, Pandur, Kühl, Behrens, von der Mark, Starzinski-Powitz, Wixler: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." dans: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).

Détails sur FHL2

Antigène: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Autre désignation: Fhl2

Sujet: The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine- rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl- 1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha