Mature Macrophages anticorps

N° du produit ABIN2192020

Cet anticorps Souris Monoclonal détecte spécifiquement Mature Macrophages dans IHC (fro), FACS, IHC (p) et WB. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 3+ publications.

Aperçu rapide pour Mature Macrophages anticorps (ABIN2192020)

Antigène: Mature Macrophages

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: 25F9

Détail du produit

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

Rosseau, Hammerl, Maus, Walmrath, Schütte, Grimminger, Seeger, Lohmeyer: "Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome." dans: American journal of physiology. Lung cellular and molecular physiology, Vol. 279, Issue 1, pp. L25-35, (2000) (PubMed).

Kiefer, Kieseier, Brück, Hartung, Toyka: "Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies." dans: Brain : a journal of neurology, Vol. 121 ( Pt 3), pp. 469-79, (1998) (PubMed).

Zwadlo, Bröcker, von Bassewitz, Feige, Sorg: "A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 3, pp. 1487-92, (1985) (PubMed).

Détail du antigène

Antigène: Mature Macrophages

Sujet: The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.