Asialoglycoprotein Receptor anticorps

N° du produit ABIN2192108

Détail du produit

Antigène: Asialoglycoprotein Receptor

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoassay (IA)

Réactivité croisée (Details): Cross reactivity: Human : Yes

Stérilité: 0.2 μm filtered

Clone: 8D7

Isotype: IgG1

Information d'application

Indications d'application: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Rat hepatocytes control Negative Rat kidney, pancreas, small intestine, colon control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Référence

Keyel, Mishra, Roth, Heuser, Watkins, Traub: "A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors." dans: Molecular biology of the cell, Vol. 17, Issue 10, pp. 4300-17, (2006) (PubMed).

Shimada, Mizuno, Uesu, Nasu, Okada, Shimomura, Yamamoto, Tsuji, Shiratori: "A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit." dans: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 26, Issue 1, pp. 55-60, (2003) (PubMed).

Hirai, Mizuno, Morisue, Yoshioka, Shimada, Nasu, Okada, Shimomura, Yamamoto, Tsuji: "Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody." dans: Acta medica Okayama, Vol. 56, Issue 3, pp. 135-9, (2002) (PubMed).

Mizuno, Yamada, Sugiyama, Vierling, Brown: "Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes." dans: Liver, Vol. 7, Issue 5, pp. 251-9, (1988) (PubMed).

Mizuno, Yamada, Nagashima: "Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes." dans: Gastroenterologia Japonica, Vol. 21, Issue 3, pp. 238-44, (1986) (PubMed).

Détail du antigène

Antigène: Asialoglycoprotein Receptor

Sujet: The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero- oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR. Aliases ASGPR 1, ASGP-R 1, Hepatic lectin 1, MHL-1 Immunogen Crude rat liver membrane extracts