C6 anticorps

N° du produit ABIN2192122

Cet anticorps Souris Monoclonal détecte spécifiquement C6 dans WB, IHC (fro) et IA. Il présente une réactivité envers Rat et a été mentionné dans 3+ publications.

Aperçu rapide pour C6 anticorps (ABIN2192122)

Antigène: C6 (Complement Component 6 (C6))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Clone: 3G11

Détail du produit anti-C6 anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-C6 (ABIN2192122)

Sato, Van Dixhoorn, Prins, Mooney, Verhagen, Muizert, Savill, Van Es, Daha: "The terminal sequence of complement plays an essential role in antibody-mediated renal cell apoptosis." dans: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 6, pp. 1242-52, (1999) (PubMed).

Baker, Ochi, Schulze, Johnson, Campbell, Couser: "Depletion of C6 prevents development of proteinuria in experimental membranous nephropathy in rats." dans: The American journal of pathology, Vol. 135, Issue 1, pp. 185-94, (1989) (PubMed).

Schulze, Baker, Perkinson, Johnson, Ochi, Stahl, Couser: "Increased urinary excretion of C5b-9 distinguishes passive Heymann nephritis in the rat." dans: Kidney international, Vol. 35, Issue 1, pp. 60-8, (1989) (PubMed).

Détails sur C6

Antigène: C6 (Complement Component 6 (C6))

Sujet: The monoclonal antibody 3G11 reacts specifically with rat C6. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 membrane attack complex. Complement component C6 is part of this C5b-9 complex. This complex is assembled from five precursor molecules in the serum. The final step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. The rat remnant kidney model had been widely used to identify mechanisms responsible for the progression of renal disease. Proteinuria is nephrotoxic and contributes to the progression of renal injury. Activation of complement in proteinuric urine results in tubular and interstitial damage. In C6- deficient rats, acute interstitial changes resolved despite ongoing proteinuria. This suggests that C6 mediates chronic progression of tubulointerstitial damage in rats with remnant kidneys. The monoclonal antibody 3G11 was raised by immunization of mice with C6 isolated from rat serum. Monoclonal antibody 3G11 can be used as detector in immuno assays detect C5b-9 in both serum and urine.