Selectin E/CD62e anticorps

N° du produit ABIN2192128

Cet anticorps Souris Monoclonal détecte spécifiquement Selectin E/CD62e dans Inhibition, IA et ISt. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour Selectin E/CD62e anticorps (ABIN2192128)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Blocking Antibody (Inhibition), Immunoassay (IA), Immunostaining (ISt)

Clone: ENA2

Détail du produit anti-Selectin E/CD62e anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100 U/mL) or LPS (1 μg/mL) in tissue culture medium at 37 °C. As negative control it is advised to use a control murine IgG1 antibody. For use in adhesion studies dilutions have to be made according to the amounts of E-Selectin to be inhibited. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Selectin E/CD62e (ABIN2192128)

Leeuwenberg, Van de Winkel, Jeunhomme, Buurman: "Functional polymorphism of IgG FcRII (CD32) on human neutrophils." dans: Immunology, Vol. 71, Issue 2, pp. 301-4, (1990) (PubMed).

Leeuwenberg, von Asmuth, Jeunhomme, Buurman: "IFN-gamma regulates the expression of the adhesion molecule ELAM-1 and IL-6 production by human endothelial cells in vitro." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 7, pp. 2110-4, (1990) (PubMed).

Leeuwenberg, Jeunhomme, Buurman: "Induction of an activation antigen on human endothelial cells in vitro." dans: European journal of immunology, Vol. 19, Issue 4, pp. 715-20, (1989) (PubMed).

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Sujet: ENA2 reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

Pathways: Thromboxane A2 Receptor Signaling