Lactoferrin, C-Lobe anticorps

N° du produit ABIN2192137

Cet anticorps Souris Monoclonal détecte spécifiquement Lactoferrin, C-Lobe dans IA et WB. Il présente une réactivité avec des échantillons de Boeuf (Vache).Il a été cité dans 1 publication.

Aperçu rapide pour Lactoferrin, C-Lobe anticorps (ABIN2192137)

Antigène: Lactoferrin, C-Lobe

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Application: Immunoassay (IA), Western Blotting (WB)

Clone: A-bC-lobe

Détail du produit

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

Shimazaki, Kamio, Nam, Harakawa, Tanaka, Omata, Saito, Kumura, Mikawa, Igarashi, Suzuki: "Structural and immunochemical studies on bovine lactoferrin fragments." dans: Advances in experimental medicine and biology, Vol. 443, pp. 41-8, (1998) (PubMed).

Détail du antigène

Antigène: Lactoferrin, C-Lobe

Sujet: Monoclonal antibody a-bC-lobe, anti bovine Lactoferrin (Lf) is highly specific for bovine Lactoferrin. This protein is a member of the transferrin family of metal-binding proteins found in milk and other secretory fluids and also in blood. It shows multifunctional properties of which the bacteriostatic and bactericidal effects are the best known. The molecule is constructed with a N-terminal half molecule (N-lobe) and a C- terminal half molecule (C-lobe), each of which is composed of two domains. The biologically important functions have been found mainly in the N-lobe. The lactoferrin determinants responsible for binding to Ca2+-dependent receptor on hepatocytes are present within the C-lobe. The monoclonal antibody a-bC- lobe shows strong reactivities with both native and denatured forms of bovine lactoferrin and C-lobe. The 'WNIPMGL' sequence (467-473 of bovine lactoferrin) is the antigenic determinant or epitopic site of the anti C-lobe antibody a-bC-lobe. The antibody shows weak reactivity with human lactoferrin and korean goat lactoferrin, slight cross reactivity is seen with bovine transferrin, whereas no cross reactivity is seen with human transferrin and chicken ovotransferrin.