Cytotoxic Necrotizing Factor 1 & 2 anticorps

N° du produit ABIN2192152

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB, DB, IA et InhA. Il présente une réactivité envers Chemical et a été mentionné dans 2+ publications.

Aperçu rapide pour Cytotoxic Necrotizing Factor 1 & 2 anticorps (ABIN2192152)

Antigène: Cytotoxic Necrotizing Factor 1 & 2

Reactivité: Chemical

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Dot Blot (DB), Immunoassay (IA), Inhibition Assay (InhA)

Clone: JC4

Détail du produit

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 or CNF2 to be inactivated.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

McNichol, Rasmussen, Carvalho, Meysick, OBrien: "Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein." dans: Infection and immunity, Vol. 75, Issue 11, pp. 5095-104, (2007) (PubMed).

Meysick, Mills, OBrien: "Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli." dans: Infection and immunity, Vol. 69, Issue 4, pp. 2066-74, (2001) (PubMed).

Détail du antigène

Antigène: Cytotoxic Necrotizing Factor 1 & 2

Classe de substances: Chemical

Sujet: The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. CNF1 and 2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal binding domain, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain. JC4 neutralizes only CNF1.