Reactivité: Humain
FACS, WB, IF, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
For immunohistology, immunfluorescence, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Date de péremption
12 months
Pelletier, Okawara, Vitale, Anderson: "Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpha+ and alpha- in guinea pig Sertoli cells: a possible association with F-actin and G-actin." dans: Biology of reproduction, Vol. 57, Issue 2, pp. 367-76, (1997) (PubMed).
Van Itallie, Balda, Anderson: "Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells." dans: Journal of cell science, Vol. 108 ( Pt 4), pp. 1735-42, (1995) (PubMed).
Balda, Anderson: "Two classes of tight junctions are revealed by ZO-1 isoforms." dans: The American journal of physiology, Vol. 264, Issue 4 Pt 1, pp. C918-24, (1993) (PubMed).
Willott, Balda, Heintzelman, Jameson, Anderson: "Localization and differential expression of two isoforms of the tight junction protein ZO-1." dans: The American journal of physiology, Vol. 262, Issue 5 Pt 1, pp. C1119-24, (1992) (PubMed).
Kurihara, Anderson, Farquhar: "Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 15, pp. 7075-9, (1992) (PubMed).
Antigène
ZONA OCCLUDENS 1 alpha-Plus
Sujet
The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain- containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and