Complement Regulator Crry anticorps

N° du produit ABIN2192227

Détail du produit

Antigène: Complement Regulator Crry

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunofluorescence (IF)

Stérilité: 0.2 μm filtered

Clone: TLD-1C11

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Référence

Fraser, Harris, Smith, Morgan: "Bacterial expression and membrane targeting of the rat complement regulator Crry: a new model anticomplement therapeutic." dans: Protein science : a publication of the Protein Society, Vol. 11, Issue 10, pp. 2512-21, (2002) (PubMed).

Hanna, Spiller, Linton, Mead, Morgan: "Rat T cells express neither CD55 nor CD59 and are dependent on Crry for protection from homologous complement." dans: European journal of immunology, Vol. 32, Issue 2, pp. 502-9, (2002) (PubMed).

McGrath, Wilkinson, Spiller, Morgan: "Development of adenovirus vectors encoding rat complement regulators for use in therapy in rodent models of inflammatory diseases." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6834-40, (2000) (PubMed).

Flaris, Densmore, Molleston, Hickey et al.: "Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal ..." dans: Glia, Vol. 7, Issue 1, pp. 34-40, (1993) (PubMed).

Détail du antigène

Antigène: Complement Regulator Crry

Sujet: The monoclonal antibody TLD-1C11 reacts with RAT COMPLEMENT regulator Crry. Complement comprises a system of soluble serum proteins that, upon activiation by antibody (classical pathway), sugars (lectin pathway), or bacterial and foreign surfaces (alternative pathway), directly causes cell damage. Complement is constantly activated at low levels in serum, requiring cells to be protect themselves from damage. In human, cells are protected from complement-mediated damage by expression of membrane-bound complement regulatory proteins, which act at serveral points along the cascade. Human cells are protected by decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and membrane attack complex inhibitor protein 1 (MIN1, CD59). Furthermore the rat analogues of DAF, MCP and CD59 have been characterized. Rats and mice express a unique membrane complement regulator of the activating pathways, called complement receptor type-1 related gene Y (Crry). In rats there are two isoforms of Crry, with six or seven short consensus repeats (SCR), encoding 65-70 and 75-85 kDa proteins, respectively, which are both transmembrane anchored. Although Crry has the functional properties of both DAF and MCP, it does not replace them. All rat blood cells express Crry whereas approximately 37 and 39 % of the rat T cells express respectively DAF and CD59. Studies in rat revealed that despite lacking DAF and CD59, these double-negative rat T cells were not more susceptible to homologous complement lysis than cells that expressed both DAF and CD59. However, blocking of the only other known rat complement regulator, Crry, enhanced lysis only at the double-negative cells. In rats, Crry exerts powerful control in the activation pathways where it acts both as a cofactor and as a decay accelerator to inactivate convertases. Crry has been succesfully expressed and refolded in bacteria. The refolded protein has full-complement regulatory activity in vitro, and has been applied in rat models of arthritis and other inflammatory diseases. The monoclonal antibody TLD-1C11 can be used to block autoimmune phenomena in rats. p65, complement receptor type-1 related gene Y Aliases rat microglial cells Immunogen Mouse IgG1