Vimentin anticorps (FITC)

N° du produit ABIN222825

Cet anticorps anti-Vimentin Monoclonal Souris conjugué à FITC (ABIN222825) détecte spécifiquement Vimentin dans WB, IHC, ELISA, IF, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Boeuf (Vache), Singe, Poulet et Chien.

Aperçu rapide pour Vimentin anticorps (FITC) (ABIN222825)

Antigène: Vimentin (VIM)

Reactivité: Humain, Boeuf (Vache), Singe, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Vimentin anticorps (FITC)

Specificité: Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57000 intermediate filament protein (vimentin) of mesenchymal cells. Reactivities on Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Species cross-reactivity: Human, monkey, bovine, canine, chicken, amphibia.

Purification: Protein A purified

Immunogène: Vimentin (purified from bovine lens).

Type of Immunogen: Purified protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Western Blot, ELISA, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT, extended with paraffin. Protease pretreatment is required prior to antibody application. Positive control: Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis