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ACTC1 anticorps (N-Term)

L’anticorps Souris Monoclonal anti-ACTC1 a été validé pour WB, IHC, ELISA, IHC (p) et IHC (fro). Il convient pour détecter ACTC1 dans des échantillons de Humain, Lapin, Poulet et Boeuf (Vache). Il y a 1 publication disponible.
N° du produit ABIN224333

Aperçu rapide pour ACTC1 anticorps (N-Term) (ABIN224333)

Antigène

Voir toutes ACTC1 Anticorps
ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivité

  • 32
  • 20
  • 18
  • 9
  • 4
  • 4
  • 2
  • 2
  • 2
Humain, Lapin, Poulet, Boeuf (Vache)

Hôte

  • 17
  • 16
  • 3
Souris

Clonalité

  • 18
  • 18
Monoclonal

Conjugué

  • 26
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp ACTC1 est non-conjugé

Application

  • 28
  • 16
  • 9
  • 8
  • 7
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Épitope

    • 7
    • 4
    • 1
    • 1
    N-Term

    Specificité

    Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue, it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species cross-reactivity: Human, bovine, rabbit, chicken.

    Purification

    Protein G purified

    Immunogène

    Synthetic N-terminus decapeptide of cardiac isoform of actin.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA (1:500), IHC, IHC-Fr, IHC-P, WB (1:1000)

    Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 1:10. Frozen and paraffin sections. Note: It is necessary to include 0.5 M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Western Blot: 1:1000 (using the ECL-enhanced procedure). ELISA: 1:500. Note: It is necessary to include 1 M sodium chloride for application in Western blot and ELISA to enhance specificity.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Sterile ddH2O

    Concentration

    Lot specific

    Buffer

    Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Aliquot to Avoid repeated freezing and thawing

    Stock

    -20 °C

    Stockage commentaire

    Reconstituted or lyophilized: store at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid freeze-thaw cycles.
  • Itier, Ret, Viale, Sweet, Bangari, Caron, Le-Gall, Bénichou, Leonard, Deleuze, Orsini: "Effective clearance of GL-3 in a human iPSC-derived cardiomyocyte model of Fabry disease." dans: Journal of inherited metabolic disease, (2014) (PubMed).

  • Antigène

    ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

    Autre désignation

    ACTC1 / Alpha Cardiac Actin

    Sujet

    Name/Gene ID: ACTC1

    Synonyms: ACTC1, Actin, alpha cardiac muscle 1, Actin, alpha, cardiac muscle, Actin, alpha, cardiac muscle 1, Alpha-cardiac actin, CMH11, CMD1R, ACTC, LVNC4, ASD5

    ID gène

    70

    UniProt

    P68032

    Pathways

    Myometrial Relaxation and Contraction
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