Laminin gamma 1 anticorps

N° du produit ABIN228737

L’anticorps Rat Monoclonal anti-Laminin gamma 1 a été validé pour IHC, IP, IF, IHC (p) et IHC (fro). Il convient pour détecter Laminin gamma 1 dans des échantillons de Humain et Souris.

Aperçu rapide pour Laminin gamma 1 anticorps (ABIN228737)

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Laminin gamma 1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A5

Détail du produit anti-Laminin gamma 1 anticorps

Specificité: Recognizes the laminin B2/g1 chain at ~210kD. Species cross-reactivity: Human and Mouse.

Purification: Protein G purified

Immunogène: Murine EHS laminin preparation.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: IF, IHC, IHC-Fr, IHC-P, IP

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Western Blot: 1-2 μg/mL for 2 hrs at RT. Immunohistochemistry (acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2 μg/mL for 30 min at RT. Note: Staining of acid-alcohol fixed tissues requires antigen unmasking with 15000U/mL of bovine testicular hyaluronidase in PBS,  pH 7.4 for 30 min at 37?C. Positive control: LS174T cells, normal colon or colon carcinoma.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur Laminin gamma 1

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Autre désignation: LAMC1 / Laminin Gamma 1

Sujet: Name/Gene ID: LAMC1
Family: Laminin

Synonyms: LAMC1, Laminin subunit gamma-1, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-1 subunit gamma, Laminin-4 subunit gamma, Laminin-9 subunit gamma, Laminin B2 chain, Laminin gamma-1 chain, Laminin-3 subunit gamma, Laminin-6 subunit gamma, S-laminin subunit gamma, Laminin Gamma 1, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, S-LAM gamma

ID gène: 3915

UniProt: P11047