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MLANA anticorps

Cet anticorps anti-MLANA est un anticorps Souris Monoclonal détectant MLANA dans WB, FACS, IHC, IHC (p) et IP. Adapté pour Humain et Cheval.
N° du produit ABIN228816

Aperçu rapide pour MLANA anticorps (ABIN228816)

Antigène

Voir toutes MLANA Anticorps
MLANA (Melan A (MLANA))

Reactivité

  • 212
  • 116
  • 91
  • 36
  • 15
  • 1
Humain, Cheval

Hôte

  • 109
  • 103
  • 1
Souris

Clonalité

  • 139
  • 74
Monoclonal

Conjugué

  • 74
  • 17
  • 9
  • 9
  • 9
  • 9
  • 9
  • 9
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp MLANA est non-conjugé

Application

  • 146
  • 110
  • 110
  • 84
  • 78
  • 53
  • 26
  • 26
  • 22
  • 21
  • 16
  • 12
  • 11
  • 9
  • 9
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • Specificité

    Recognizes MART-1 at 18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Species cross-reactivity: Human and equine. Does not cross-react with mouse or rat.

    Purification

    Protein A purified

    Immunogène

    Recombinant hMART-1 protein. MW: 18kD. Cellular Localization: Cytoplasmic.

    Type of Immunogen: Recombinant protein

    Isotype

    IgG2b kappa
  • Indications d'application

    Approved: Flo, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

    Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein A). Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: CaC1 melanoma cells and melanomas.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % BSA, 0.05 % sodium azide, 40 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    MLANA (Melan A (MLANA))

    Autre désignation

    MLANA / Melan-A / MART-1

    Sujet

    Name/Gene ID: MLANA

    Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1

    ID gène

    2315

    UniProt

    Q16655
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