MHV NSP9 anticorps
Aperçu rapide pour MHV NSP9 anticorps (ABIN233751)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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N° du produit (Fournisseur)
- 200-301-a56
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Fournisseur
- Rockland
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Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
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Immunogène
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This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native -
Isotype
- IgG
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Indications d'application
- This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.1 mg/mL
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C
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- MHV NSP9
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Classe de substances
- Viral Protein
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Sujet
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The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody -
ID gène
- 1489749, 25121567
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UniProt
- P0C6V0
Antigène
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