MHV NSP9 anticorps

N° du produit ABIN233751

Détail du produit

Antigène: MHV NSP9

Reactivité: Mouse Hepatitis Virus (MHV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native

Clone: 2C6-H1

Isotype: IgG

Information d'application

Indications d'application: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA.  After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détail du antigène

Antigène: MHV NSP9

Classe de substances: Viral Protein

Sujet: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody

ID gène: 1489749, 25121567

UniProt: P0C6V0