UBE2J1 anticorps (N-Term)

N° du produit ABIN233822

N° du produit (Fournisseur): 600-401-991

L’anticorps Lapin Polyclonal anti-UBE2J1 a été validé pour WB, ELISA et IP. Il convient pour détecter UBE2J1 dans des échantillons de Humain.

Aperçu rapide pour UBE2J1 anticorps (N-Term) (ABIN233822)

Antigène: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2J1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-UBE2J1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-991

Fournisseur: Rockland

Fonction: UBE2J1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Ube2j1 protein.

Attributs du produit: Synonyms: rabbit anti-UBE2J1 antibody, rabbit anti-E2 J1 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J1 antibody, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1-2 μg

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.71 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur UBE2J1

Antigène: UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Autre désignation: UBE2J1

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain.  By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response.

ID gène: 51465, 37577122

UniProt: Q9Y385