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CD15 (Granulocytes) anticorps (Biotin)

L’anticorps Souris Monoclonal anti- a été validé pour FACS, IHC (f) et IFA. Il convient pour détecter dans des échantillons de Humain.
N° du produit ABIN234527

Aperçu rapide pour CD15 (Granulocytes) anticorps (Biotin) (ABIN234527)

Antigène

CD15 (Granulocytes)

Reactivité

Humain

Hôte

  • 1
Souris

Clonalité

Monoclonal

Conjugué

  • 1
Biotin

Application

Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Indirect Immunofluorescence Assay (IFA)

Clone

TG1
  • Specificité

    Recognizes a (Mr 156 kDa) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes.(1,2,3) The CD15 antigen is expressed on approximately 95% of human peripheral blood eosinophils and neutrophils and is present on approximately 20% of mature circulating monocytes. Anti-CD15 has virtually no reactivity with T or B cells.(1,2,3)

    Attributs du produit

    MAb to CD15 Granulocytes, Monoclonal antibody to CD15 (Granulocytes) Biotin conjugated

    Purification

    Ion Exchange Chromatography and gel filtration. Tissue culture

    Immunogène

    Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1M glucose

    Isotype

    IgM
  • Indications d'application

    Suitable for use in flow cytometry, IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    0.01 M PBS, pH7.2 containing 1 % BSA, 0.1 % Sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    DO NOT FREEZE.
    Centrifuge product if not completely clear after standing at room temperature.
    Prepare working dilution only prior to immediate use.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8 °C.
  • Antigène

    CD15 (Granulocytes)
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