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RAB11A anticorps (C-Term)

RAB11A Reactivité: Humain WB, ELISA, IHC, IF, IP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2346690
  • Antigène Voir toutes RAB11A Anticorps
    RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
    Épitope
    • 9
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 2
    • 1
    • 1
    C-Term
    Reactivité
    • 51
    • 25
    • 23
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 40
    • 8
    • 5
    Lapin
    Clonalité
    • 45
    • 8
    Polyclonal
    Conjugué
    • 32
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RAB11A est non-conjugé
    Application
    • 40
    • 24
    • 10
    • 8
    • 5
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
     Réactivité croisée
    Chien, Humain, Lapin, Rat (Rattus)
    Réactivité croisée (Details)
    Calculated cross reactivity: Ca Hu Rb Rt
    Attributs du produit
    Rab11A (RAB11A Member RAS Oncogene Family, Rab-11A, MGC1490, RAB11, Rab-11, Ras-related Protein Rab-11A, Ras-related protein Rab11A, YL8)
    Purification
    Purified by immunoaffinity chromatography.
    Immunogène
    Synthetic peptide corresponding to the C-terminal hypervariable region of the human Rab11a sequence. Sequence Homology: Rab11b protein-10/17.
    Isotype
    IgG
    Top Product
    Discover our top product RAB11A Anticorps primaire
  • Indications d'application
    Optimal working conditions should be determined by the investigator.
    Restrictions
    For Research Use only
  • Validation #101022 (Western Blotting)
    'Independent Validation' signe
    by
    Epidermis Differentiation and Rheumatoid Autoimmunity (INSERM U1056 UDEAR), University of Toulouse
    No.
    #101022
    Date
    27.07.2017
    Antigène
    RAB11A
    Numéro du lot
    L16102562
    Application validée
    Western Blotting
    Contrôle positif

    Cell lysates from total human epidermis

    Cell lysates from reconstructed human epidermis transfected with control shRNA

    Contrôle négative

    Cell lysates from reconstructed human epidermis transfected with RAB11A shRNA

    Conclusion
    Passed. ABIN2346690 specifically recognizes Rab11a in human epidermal samples.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2346690
    Anticorps secondaire
    goat anti-rabbit Ig HRP-conjugated antibody (Southern Biotech, 4030-05, lot J1810)
    Full Protocol
    • Obtain total human epidermis from abdominal dermolipectomy and treat with dispase (Sigma-Aldrich, D4693) to dissociate it from the dermis.
    • For production of reconstructed human epidermis, obtain isolated keratinocytes from epidermal fragments. Grow cells in Epilife (Invitrogen Life Technologies, MEPICF500, lot 1838688) supplemented with penicillin and streptomycin (Sigma-Aldrich, P4333, lot 1868847), HKGS (Human Keratinocyte Growth Supplement, ThermoFisher Scientific, S0015, lot 1848268), 1.5mM calcium (Sigma-Aldrich 25011), KGF (Keratinocyte Growth Factor, Sigma-Aldrich, K1767, lot MKBX7825V) and vitamin C (Sigma-Aldrich, 49752) on polycarbonate culture inserts (Merck Millipore, PIHP01250) at 37°C and 5% CO2, according to Reynier et al. (2016).
    • For silencing with interfering shRNA, transduce keratinocytes with MISSION pLKO.1 puro vector-based lentiviral particles containing a puromycin resistance gene and an shRNA insert under the human U6 promoter (Sigma-Aldrich, TRCN0000303291 for Rab11A and SHC002 for the negative control) following the manufacturer´s instructions, before cell seeding for production of reconstructed human epidermis.
    • Lyse normal or reconstructed epidermis in 300µL/0.6cm2 1x Laemmli buffer (Tris-HCl 63mM pH6.8, SDS 2%, Glycerol 10%, Bromophenol blue 0.0005%, DTT 1mM, all Sigma-Aldrich) and heat at 95°C for 5min.
    • Separate 5µL of total protein extracts in 1x Laemmli buffer on denaturing 10% polyacrylamide SDS gels in a Mini-Protean Tetra Cell electrophoresis cell system (Bio-Rad , 1658004) at 100V for 90min.
    • Transfer proteins onto Hybond ECL membrane (GE Healthcare, RPN3032D) with a Mini TransBlot Western blotting system (Bio-Rad, 1658029) at 100V for 90 min.
    • Block membrane with 1x PBS containing Tween 20 (Sigma-Aldrich) at 0.05% (PBST) and 5% low fat milk at 3% at RT for 1h.
    • Incubate the membrane with primary rabbit anti-human RAB11A, Member RAS Oncogene Family (RAB11A) (C-Term) antibody (antibodies-online, ABIN2346690, lot L16102562) diluted 1:250 in blocking buffer ON at 4°C.
    • Wash the membrane 3x for 15min with 1x PBST.
    • Incubate the membrane with secondary goat anti-rabbit Ig HRP-conjugated antibody (Southern Biotech, 4030-05, lot J1810) diluted 1:10000 in blocking buffer for 90min at RT.
    • Wash the membrane 3x for 15min with 1x PBST.
    • Reveal protein bands using ECL prime Detection Reagent (GE Healthcare, RPN2238, lot 9838391) on a GBox apparatus (Syngene).
    Notes
  • Format
    Liquid
    Buffer
    Supplied as a liquid in PBS, pH 7.4, 0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Antigène
    RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
    Autre désignation
    Rab11A (RAB11A Produits)
    Synonymes
    anticorps rab11, anticorps RAB11A, anticorps wu:fi15h09, anticorps zgc:103679, anticorps yl8, anticorps Rab11a, anticorps rab11A, anticorps DDBDRAFT_0190819, anticorps DDBDRAFT_0191190, anticorps DDB_0190819, anticorps DDB_0191190, anticorps YL8, anticorps RAB11, anticorps RAB11A, member RAS oncogene family S homeolog, anticorps RAB11A, member RAS oncogene family, anticorps RAB11a, member RAS oncogene family, anticorps Rab11 GTPase, anticorps rab11A protein, anticorps Rab11a, GTPase, anticorps Rab GTPase, anticorps rab11A, RAB family GTPase, anticorps rab11a.S, anticorps RAB11A, anticorps rab11a, anticorps Rab11a, anticorps rab11A
    Pathways
    Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity
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