Shows only the RNP precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong RNP precipitate band in double diffusion. No reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Scl-70, Sm, and Jo-1 antigens in an ELISA system designed to detect all IgG subclasses.
Attributs du produit
Human anti Human RNP antigen Human antibody to Human RNP antigen
Purification
Single donor human plasma is delipidized and defibrinated. An immunoglobulin fraction is precipitated and collected. Human plasma.
Reactivité: Humain
ID, EIA, WB
Hôte: Human
Polyclonal
unconjugated
Indications d'application
Suitable for use in double immunodiffusion and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute with 2 mL of deionized water
Buffer
Lyophilized from 0.01 M Phosphate, 0.11 M Sodium chloride, pH 7.2, 0.02 % Sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.