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Orf1 anticorps

L’anticorps Lapin Polyclonal anti- a été validé pour WB. Il convient pour détecter dans des échantillons de Neisseria meningitidis.
N° du produit ABIN238346

Aperçu rapide pour Orf1 anticorps (ABIN238346)

Antigène

Orf1

Reactivité

Neisseria meningitidis

Hôte

  • 4
Lapin

Clonalité

  • 4
Polyclonal

Conjugué

  • 1
  • 1
  • 1
  • 1
Inconjugué

Application

Western Blotting (WB)
  • Purification

    Protein-A affinity chromatography.

    Immunogène

    Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
  • Indications d'application

    Suitable for Western Blotting under Reducing conditions (0.2-1 μg/mL).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4 with 15 mM sodium azide as preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    Orf1

    Classe de substances

    Viral Protein

    Sujet

    Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
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