Collagen Type I anticorps

N° du produit ABIN238355

L’anticorps Lapin Polyclonal anti-Collagen Type I a été validé pour IHC (fro), IHC (p), IF, RIA et EIA. Il convient pour détecter Collagen Type I dans des échantillons de Boeuf (Vache).

Aperçu rapide pour Collagen Type I anticorps (ABIN238355)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Détail du produit anti-Collagen Type I anticorps

Specificité: Bovine Collagen type I: 100 % . Bovine Collagen II, IX, XI: < 0.1 % Sheep Collagen type I: 50 % (IHC estimated) Bovine Fibronectin: < 0.1 % Human Collagen type I: < 2 % Chicken Collagen type I: < 0.1 % (Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500). Even though cross-reactivity with human is low in RIA, the antibody has to be expected to be cross-reactiv to human Collagen type I as well as to pig Collagen type I in IHC.

Réactivité croisée (Details): Species reactivity (tested):Bovine. Cross reacts with Sheep.

Purification: Affinity Chromatography

Immunogène: Purified Collagen type I from Bovine skin

Information d'application

Indications d'application: RIA. ELISA: 1/400 (OD > 500). Immunoblotting (Western blot). Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissues: 1/500-1/1000. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C overnight. Positive Control: Bovine skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives, None, None

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I

Sujet: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

ID gène: 282187

NCBI Accession: NP_001029211

UniProt: P02453