Collagen Type I anticorps

N° du produit ABIN238356

Cet anticorps anti-Collagen Type I est un anticorps Lapin Polyclonal détectant Collagen Type I dans IHC (fro), IHC (p), IF, RIA et EIA. Adapté pour Poulet.

Aperçu rapide pour Collagen Type I anticorps (ABIN238356)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Détail du produit anti-Collagen Type I anticorps

Purification: Affinity chromatography

Immunogène: Purified collagen type I from chicken skin

Information d'application

Indications d'application: Immunflourescence 1: 40-1: 80. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections 1: 500 - 1: 1000 (please see also Protocols). Incubation time: 60 min at RT or 2-8 °C over night. Positive control: chicken skin. ELISA. RIA 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Pretreatment of tissue: After de-waxing the tissue slices are treated with 0. 2% hyaluronidase (app. 300 U/mg) in

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL aqua destilled, for further dilution use appropriate antibody diluent.

Concentration: app. 1 mg/mL

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I

Sujet: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

ID gène: 9031

UniProt: P02457