Collagen Type I anticorps

N° du produit ABIN238357

Cet anticorps Lapin Polyclonal détecte spécifiquement Collagen Type I dans IHC (fro), IF, IHC (p), RIA et EIA. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour Collagen Type I anticorps (ABIN238357)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Détail du produit anti-Collagen Type I anticorps

Specificité: Rat Collagen type I: 100 % Rat Collagen II, III and V: < 0.1 % Human, Mouse, Chicken Collagen type I: < 0.1 % Rat Elastin: < 0.1 % (determined by RIA at 1/200 dilution).

Purification: Affinity Chromatography

Immunogène: Purified Collagen type I from Rat skin

Information d'application

Indications d'application: ELISA: >/= 1: 200. RIA. Immunofluorescence: 1: 80. Immunohistochemistry on paraffin sections: 1: 500 (60 min at RT or 2-8C over night). Positive control: Rat skin. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS, None, None

Précaution d'utilisation: Do not use Sodium Azide as Preservative.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I

Sujet: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is mainly found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

ID gène: 29393

NCBI Accession: NP_445756

UniProt: P02454