ACRV1 anticorps

N° du produit ABIN238420

Cet anticorps Souris Monoclonal détecte spécifiquement ACRV1 dans WB, IF et FACS. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour ACRV1 anticorps (ABIN238420)

Antigène: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACRV1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Hs-14

Détail du produit anti-ACRV1 anticorps

Specificité: The antibody Hs-14 reacts with a 220 kDa testis-specific intra-acrosomal protein associated with the membranes of the acrosomal vesicle.

Attributs du produit: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Purification: Precipitation methods and size-exclusion chromatography

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Isotype: IgM

Information d'application

Indications d'application: Flow Cytometry. Western Blotting. Immunocytochemistry: 10 μg/mL, Staining technique: Membrane permeabilization(acetone) is essential. Application note: The antibody Hs-14 is designed for quantitativeimmunofluorescence analysis of pathological sperms (excellent tool for laboratories ofassisted reproduction when optimal method of fertilization is sought).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody at 2 - 8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur ACRV1

Antigène: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Autre désignation: ACRV1

Sujet: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

ID gène: 56

UniProt: P26436