TUBG1 anticorps (C-Term)

N° du produit ABIN238522

Cet anticorps Souris Monoclonal détecte spécifiquement TUBG1 dans WB et IF. Il présente une réactivité envers Humain, Rat, Souris, Poulet, Plantes, Protozoa et Porc.

Aperçu rapide pour TUBG1 anticorps (C-Term) (ABIN238522)

Antigène: TUBG1 (Tubulin, gamma 1 (TUBG1))

Reactivité: Humain, Rat, Souris, Poulet, Plantes, Protozoa, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBG1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TU-30

Détail du produit anti-TUBG1 anticorps

Épitope: AA 434-449, C-Term

Specificité: The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.

Attributs du produit: Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

Purification: Protein-A Affinity Chromatography

Immunogène: C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry (Purified Antibody: 1-2 μg/mL, Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly inmethanol for 10 min at -20C or in acetone for 10 min at -20C. Incubation: 45 min in roomtemperature, Positive control: P-19 mouse embryonal carcinoma cell line 3T3 mousefibroblasts, Application note: The antibody TU-30 stains only fixed cells). Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur TUBG1

Antigène: TUBG1 (Tubulin, gamma 1 (TUBG1))

Autre désignation: TUBG1 / Tubulin gamma 1

Sujet: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

ID gène: 7283

UniProt: P23258

Pathways: Dynamique des Microtubules, M Phase