NCEH1 anticorps (C-Term)

N° du produit ABIN238671

Cet anticorps anti-NCEH1 est un anticorps Chèvre Polyclonal détectant NCEH1 dans ELISA, IF et FACS. Adapté pour Humain.

Aperçu rapide pour NCEH1 anticorps (C-Term) (ABIN238671)

Antigène: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NCEH1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-NCEH1 anticorps

Épitope: C-Term

Fonction: AADACL1

Séquence: RTRNSYIKWL DQN

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary experiments gave an approx 26-28 kDa band in A431 cell lysates and in Human Hippocampus lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:1000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended con

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NCEH1

Antigène: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Autre désignation: AADACL1

Sujet: AADACL1, arylacetamide deacetylase-like 1

ID gène: 57552, 320024

NCBI Accession: NP_065843, NP_001139748, NP_001139749