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ODF2 anticorps

ODF2 Reactivité: Humain, Rat, Souris WB, ELISA, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2430583
  • Antigène Voir toutes ODF2 Anticorps
    ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))
    Reactivité
    • 30
    • 20
    • 13
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain, Rat, Souris
    Hôte
    • 43
    • 2
    • 1
    Lapin
    Clonalité
    • 43
    • 3
    Polyclonal
    Conjugué
    • 18
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp ODF2 est non-conjugé
    Application
    • 31
    • 13
    • 13
    • 11
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purification
    Affinity purification
    Immunogène
    Recombinant protein of human ODF2
    Isotype
    IgG
  • Indications d'application
    WB 1:500-1:2000, IHC 1:25-1:100
    Restrictions
    For Research Use only
  • Validation #100023 (Western Blotting)
    'Independent Validation' signe
    by
    Georg-August-University of Göttingen, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Developmental Biology
    No.
    #100023
    Date
    03.05.2016
    Antigène
    Outer Dense Fiber of Sperm Tails 2 (ODF2)
    Numéro du lot
    Application validée
    Western Blotting
    Contrôle positif
    recombinant ODF2-His, bacterially expressed; NIH3T3 total cell lysate
    Contrôle négative
    Conclusion
    Passed, regarding sensitivity and specificity. The antibody detects bacterially expressed ODF2 (see 2)) and the endogenous protein (see 3)) with only moderate background staining in WB.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2430582
    Anticorps secondaire
    anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450)
    Full Protocol
    • Proteins separated on 10% SDS-PAGE (Laemmli 1970), blotted to Protran (GE Healthcare No. 10600004, 0.2 µm) (Towbin et al., 1979). Blocking of membrane in 5% skim milk in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 30 min at room temperature.
    • Incubation with first antibody in 5% skim milk in TBST at 4°C overnight.
    • Washing in TBST for 30 minutes at room temperature.
    • Incubation with secondary antibody in 5% skim milk in TBST for 45 minutes at room temperature. Washing in TBST for 30-45 minutes at room temperature.
    • Secondary antibody: anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450). Dilution: 1:10,000.
    • Chemiluminescence detection (Western Lightning Ultra, Lot 215-15091, Perkin Elmer) and image capture via BioRad Chemdoc or X-ray films.
    Notes
  • Validation #100024 (Immunocytochemistry)
    'Independent Validation' signe
    by
    Georg-August-University of Göttingen, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Developmental Biology
    No.
    #100024
    Date
    03.05.2016
    Antigène
    Outer Dense Fiber of Sperm Tails 2 (ODF2)
    Numéro du lot
    Application validée
    Immunocytochemistry
    Contrôle positif
    mouse anti-γ-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808)
    Contrôle négative
    Conclusion
    Passed, the antibody detects centrosomal ODF2 in immunocytological preparations with only very low background staining (the green dots are mostly the correct location of ODF2 as they overlap with γ-tubulin spots (in red)).
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2430582
    Anticorps secondaire
    MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008)
    Full Protocol
    • NIH3T3 cells (ATCC) were grown on cover slips in DMEM, 10% fetal bovine serum, 5% penicillin/streptomycin (all Gibco) at 37°C in 5% CO2.
    • Cells were fixed in ice-cold methanol for 20 min at -20°C.
    • Unspecific binding sites were blocked in PBST (phosphate buffered saline (PBS) containing 0.15% bovine serum albumin, 0.1% Tween-20) for 1 hr.
    • Cells were incubated with the primary antibodies at 4°C overnight.
    • Cells were washed in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 15 min.
    • Cells were incubated with secondary antibodies and DAPI for 1h at 37°C.
    • Cells were washed in TBST for 15 min.
    • Cells were embedded in Dako Fluorescent Mounting Medium (Dako).
    • As positive control for centrosomal decoration anti ?-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808; 1:100) was used. anti ?-tubulin was detected with MFP590 goat anti-mouse IgG (H+L) (MoBiTec, #MFP-A1032; 1:300).
    • Anti ODF2-antibody was detected with MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008; 1:300).
    • DNA was counterstained with DAPI.
    • Images were taken by confocal microscopy (LSM 510, Zeiss), and processed using Adobe Photoshop 5.0.
    Notes
  • Format
    Liquid
    Concentration
    0.3 mg/mL
    Buffer
    PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4
    Agent conservateur
    Sodium azide
    Conseil sur la manipulation
    Avoid freeze / thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C. Avoid freeze / thaw cycles.
  • Rahimi, Cai, Kılıҫ, Kazerouni, Tapia Contreras, Hoyer-Fender: "Expression of α-Tubulin Acetyltransferase 1 and Tubulin Acetylation as Selective Forces in Cell Competition." dans: Cells, Vol. 10, Issue 2, (2021) (PubMed).

    Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." dans: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

  • Antigène
    ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))
    Autre désignation
    ODF2 (ODF2 Produits)
    Synonymes
    anticorps odf84, anticorps odf2/1, anticorps odf2/2, anticorps DKFZp469J0214, anticorps CT134, anticorps ODF2/1, anticorps ODF2/2, anticorps ODF84, anticorps si:dkey-228b2.4, anticorps AI848335, anticorps MMTEST29, anticorps outer dense fiber of sperm tails 2, anticorps outer dense fiber of sperm tails 2a, anticorps outer dense fiber of sperm tails 2 L homeolog, anticorps ODF2, anticorps odf2, anticorps odf2a, anticorps odf2.L, anticorps Odf2
    Sujet
    The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants.
    Poids moléculaire
    Calculated MW: 95 kDa
    Pathways
    M Phase
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