GFP anticorps

N° du produit ABIN243767

L’anticorps anti-GFP Polyclonal Lapin est utilisé pour la détection de GFP dans des échantillons de Aequorea victoria. Il a été validé pour WB, IHC, IF, IP, ICC et IHC (p).

Aperçu rapide pour GFP anticorps (ABIN243767)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GFP anticorps

Specificité: Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP, S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.

Purification: Affinity purified

Immunogène: Highly purified recombinant GFP made in E. coli.

Isotype: IgG

Information d'application

Indications d'application: Approved: ICC (1:200 - 1:1000), IF, IHC, IHC-P (1:2500 - 1:10000), IP, WB (1:2500 - 1:10000)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3 % BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4 % normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13 min in 10 mM citrate buffer  pH 6 followed by rapid cooling on ice). Immunoprecipitation: 1 μL per 10cm tissue culture dish of transfected cells. Use 10 μL protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100.

Commentaires:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212