anti-HRP anticorps

N° du produit ABIN2443813

Cet anticorps Souris Monoclonal détecte spécifiquement HRP dans IHC. Il présente une réactivité avec des échantillons de Raifort.

Aperçu rapide pour anti-HRP anticorps (ABIN2443813)

Antigène: HRP (Horseradish Peroxidase (HRP))

Reactivité: Raifort

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (IHC)

Clone: MAP A6-2

Détail du produit anti-HRP anticorps

Fonction: Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)

Specificité: A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein

Purification: Protein G purified

Immunogène: HRP protein

Isotype: IgG1

Information d'application

Indications d'application: IHC 1:30

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur HRP

Sujet: Horseradish peroxidase (HRP) is a 40 kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.