beta Actin anticorps

N° du produit ABIN2443850

L’anticorps anti-beta Actin Monoclonal Souris est utilisé pour la détection de beta Actin dans des échantillons de Humain, Souris, Rat, Poulet et Lapin. Il a été validé pour WB, ELISA, IHC et DB.

Aperçu rapide pour beta Actin anticorps (ABIN2443850)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Souris, Rat, Poulet, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Clone: BA3R

Détail du produit anti-beta Actin anticorps

Fonction: Beta-Actin Mouse Monoclonal Antibody (BA3R)

Specificité: The antibody recognizes native and denatured forms of β-Actin (~42 kDa)

Purification: Protein G purified

Isotype: IgG2b

Information d'application

Indications d'application: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: Beta-Actin

Sujet: β-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The β-actin gene is often stably and constitutively expressed at high levels in most tissues and cells. Proteins such as β-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction