VEGF anticorps

N° du produit ABIN2443873

Cet anticorps Poulet Polyclonal détecte spécifiquement VEGF dans WB et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Porc.

Aperçu rapide pour VEGF anticorps (ABIN2443873)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Humain, Souris, Porc

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-VEGF anticorps

Fonction: VEGF Chicken Polyclonal Antibody

Specificité: The antibody recognizes the partially reduced homodimmers and multimers that VEGF spontaneously forms. In reduced samples, the antibody recognizes the reduced monomer.

Purification: Purified

Immunogène: Recombinant human VEGF

Information d'application

Indications d'application: WB 1:1000,IF 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 30 μL: WB 1:1000 - 1:8000, IF 1:500 - 1:2000 (in cells fixed with paraformaldehyde). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified total IgY in PBS pH 7.4

Agent conservateur: Sodium azide, Streptomycin

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Autre désignation: VEGF

Sujet: Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that stimulates vasculogenesis and angiogenesis. VEGF belongs to a family of closely related growth factors that share a conserved pattern of eight cysteine residues and bind to common VEGF receptors. The VEGF family members bind in an overlapping manner to three receptor tyrosine kinases, VEGFR1, VEGFR2 and VEGFR3. Specifically, VEGF mediates angiogenic response by the activation of VEGFR2. VEGF is produced at high levels in endothelial cells but its expression can be found in other cell types, including fibroblasts, inflammatory cells, and many tumor cells, often in response to increasing tumor hypoxia via the HIF-1a pathway. Overexpression of VEGF can contribute to diseases such as cancer and vascular disease in the retina, also, VEGF is a cause of susceptibility to microvascular complications of diabetes type 1 such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy.

UniProt: P15692