EpCAM anticorps

N° du produit ABIN2443916

L’anticorps anti-EpCAM Monoclonal Souris est utilisé pour la détection de EpCAM dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC et ELISA.

Aperçu rapide pour EpCAM anticorps (ABIN2443916)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 4A7

Détail du produit anti-EpCAM anticorps

Fonction: Epcam ICD Mouse Monoclonal Antibody (4A7)

Specificité: The antibody recognizes the intracellular domain of human EpCAM.

Purification: Protein G purified

Isotype: IgG1

Information d'application

Indications d'application: WB 1:1000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Sujet:

Epithelial Cell Adhesion Molecule Intracellular Domain

Epithelial cell adhesion molecule (EpCAM) is a 40- kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

UniProt: P16422