Clathrin, Light Chain (N-Term) anticorps

N° du produit ABIN2443922

L’anticorps anti-Clathrin, Light Chain Polyclonal Lapin est utilisé pour la détection de Clathrin, Light Chain dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IF.

Aperçu rapide pour Clathrin, Light Chain (N-Term) anticorps (ABIN2443922)

Antigène: Clathrin, Light Chain

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit

Épitope: N-Term

Fonction: Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody

Specificité: The antibody recognizes the N-terminal portion of Clathrin Light Chain

Purification: Immunogen affinity purified

Immunogène: peptide in N term

Information d'application

Indications d'application: IF 1:100,WB 1:1000-1:2000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 200 μL: IF 1:100 to 1:500, WB 1:1000-1:2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détail du antigène

Antigène: Clathrin, Light Chain

Autre désignation: Clathrin Light Chain

Sujet:

Clathrin light chain A, Lca

Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.

UniProt: P09496