Leishmania A2 protein (A2) anticorps

N° du produit ABIN2443977

Cet anticorps anti-Leishmania A2 protein Monoclonal Souris (Clone C9) (ABIN2443977) détecte spécifiquement Leishmania A2 protein dans IF, IP et WB. L’anticorps est réactif avec des échantillons de Leishmania donovani (L. donovani) et Leishmania.

Aperçu rapide pour Leishmania A2 protein (A2) anticorps (ABIN2443977)

Antigène: Leishmania A2 protein (A2)

Reactivité: Leishmania donovani (L. donovani), Leishmania

Hôte: Souris

Clonalité: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Clone: C9

Détail du produit

Fonction: Leishmania A2 Mouse Monoclonal Antibody (C9)

Séquence: QSVSVGPGPL

Specificité: A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence can also be used as tag if cloned with the protein of interest. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. There is no cross-reactivity with other Leishmania species.

Purification: Protein G purified

Immunogène: The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.

Isotype: IgG1

Information d'application

Indications d'application: WB 1:100

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détail du antigène

Antigène: Leishmania A2 protein (A2)

Autre désignation: Leishmania A2

Sujet: Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.

UniProt: A4HZU7