HIV-1 Nef (full length) anticorps

N° du produit ABIN2452025

Détail du produit

Antigène: HIV-1 Nef

Épitope: full length

Reactivité: Human Immunodeficiency Virus (HIV)

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Attributs du produit: Whole Antiserum

Purification: Guinea Pig serum

Immunogène: Purified full-size recombinant Nef of HIV-1 subtype B expressed in E. coli

Information d'application

Indications d'application: 1. Western blot: 1/1,000~1/3,000
2. Dot blot: 1/3000
3. Immunoprecipitation (assay dependent)
4. ELISA (assay dependent)
Other applications have not been tested

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Upon receipt aliquot and store at -20 C

Référence

Arora, Fredericksen, Garcia: "Nef: agent of cell subversion." dans: Microbes and infection / Institut Pasteur, Vol. 4, Issue 2, pp. 189-99, (2002) (PubMed).

Fackler, Baur: "Live and let die: Nef functions beyond HIV replication." dans: Immunity, Vol. 16, Issue 4, pp. 493-7, (2002) (PubMed).

Détail du antigène

Antigène: HIV-1 Nef

Classe de substances: Viral Protein

Sujet: HIV-1 Nef (27 kDa) is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.