MID1 anticorps

N° du produit ABIN2461022

L’anticorps Lapin Polyclonal anti-MID1 a été validé pour WB et ELISA. Il convient pour détecter MID1 dans des échantillons de Humain, Souris, Rat, Chien et Poisson zèbre (Danio rerio).

Aperçu rapide pour MID1 anticorps (ABIN2461022)

Antigène: MID1 (Midline 1 (MID1))

Reactivité: Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MID1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-MID1 anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MID1.

Information d'application

Indications d'application: MID1 antibody can be used for detection of MID1 by ELISA at 1:312500. MID1 antibody can be used for detection of MID1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MID1 antibody at -20 °C.

Détails sur MID1

Antigène: MID1 (Midline 1 (MID1))

Autre désignation: MID1

Sujet: MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing, however, the full length nature of two variants has not been determined.

Poids moléculaire: 75 kDa, 75 kDa, 62 kDa

ID gène: 4281

NCBI Accession: NP_000372

UniProt: O15344