ZNF322A anticorps
Aperçu rapide pour ZNF322A anticorps (ABIN2461271)
Antigène
Voir toutes ZNF322A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by peptide affinity chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF322A.
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Indications d'application
- ZNF322A antibody can be used for detection of ZNF322A by ELISA at 1:312500. ZNF322A antibody can be used for detection of ZNF322A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZNF322A antibody at -20 °C.
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- ZNF322A (Zinc Finger Protein 322A (ZNF322A))
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Autre désignation
- ZNF322A
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Sujet
- ZNF322 contains four exons and spans 23.2kb in chromosome 6p22.1 region, and transcribes a 2.7kb mRNA that encodes a protein with 402 amino acid residues.Through northern blot analysis, ZNF322 was shown to be expressed in every human tissue examined at adult stage and during embryonic developmental stages from 80 days to 24 weeks. When ZNF322 was overexpressed in COS-7 cells, ZNF322-EGFP fusion protein is detected in the nucleus and cytoplasm. Reporter gene assays show that ZNF322 is a transcriptional activator. Furthermore, overexpression of ZNF322 in COS-7 cells activates the transcriptional activity of SRE and AP-1. Together, these results suggest that ZNF322 is a member of the zinc-finger transcription factor family and may act as a positive regulator in gene transcription mediated by the MAPK signaling pathways.
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Poids moléculaire
- 47 kDa, 47 kDa
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ID gène
- 79692
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NCBI Accession
- NP_078915
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UniProt
- Q6U7Q0
Antigène
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