L’anticorps anti-EIF4A3 Polyclonal Lapin est utilisé pour la détection de EIF4A3 dans des échantillons de Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio) et C. elegans. Il a été validé pour WB et ELISA.
EIF4A3
Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Cheval, Porc
IHC, IF, IP, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
DDX48 antibody can be used for detection of DDX48 by ELISA at 1:312500. DDX48 antibody can be used for detection of DDX48 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Conseil sur la manipulation
As with any antibody avoid repeat freeze-thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX48 antibody at -20 °C.
DDX48 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.