E2F3 anticorps

N° du produit ABIN2461642

Cet anticorps Lapin Polyclonal détecte spécifiquement E2F3 dans WB et ELISA. Il présente une réactivité envers Humain, Souris, Rat et Chien.

Aperçu rapide pour E2F3 anticorps (ABIN2461642)

Antigène: E2F3

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-E2F3 anticorps

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F3.

Information d'application

Indications d'application: E2F3 antibody can be used for detection of E2F3 by ELISA at 1:62500. E2F3 antibody can be used for detection of E2F3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store E2F3 antibody at -20 °C.

Détails sur E2F3

Antigène: E2F3

Sujet: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Poids moléculaire: 37 kDa, 49 kDa

ID gène: 1871

UniProt: Q68DT0