UPF3B anticorps

N° du produit ABIN2462297

L’anticorps anti-UPF3B Polyclonal Lapin est utilisé pour la détection de UPF3B dans des échantillons de Humain et Chien. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour UPF3B anticorps (ABIN2462297)

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Reactivité: Humain, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UPF3B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-UPF3B anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UPF3B.

Information d'application

Indications d'application: UPF3B antibody can be used for detection of UPF3B by ELISA at 1:312500. UPF3B antibody can be used for detection of UPF3B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store UPF3B antibody at -20 °C.

Détails sur UPF3B

Antigène: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Autre désignation: UPF3B

Sujet: UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Poids moléculaire: 52 kDa, 58 kDa

ID gène: 65109

NCBI Accession: NP_075386

UniProt: Q9BZI7