Aperçu rapide pour DEFA1/3 (Internal Region) anticorps (ABIN2464584)
Antigène
DEFA1/3
Reactivité
Humain
Hôte
Chèvre
Clonalité
Polyclonal
Conjugué
Inconjugué
Application
ELISA, Western Blotting (WB)
Épitope
Internal Region
Specificité
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Purification
DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.
Reactivité: Humain
Hôte: Lapin
Polyclonal
Alexa Fluor 750
Indications d'application
ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.