MLF1IP anticorps (Thr78)

N° du produit ABIN2470179

Détail du produit anti-MLF1IP anticorps

Antigène: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Épitope: Thr78

Reactivité: Humain, Boeuf (Vache), Chien, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MLF1IP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificité: PThr78

Purification: Immunoaffinity Chromatography

Immunogène: MLF1IP antibody was raised against amino acids surrounding Thr78 of MLF1IP (Human).

Information d'application

Indications d'application: MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies avoid freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store MLF1IP antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 °C or below.

Détails sur MLF1IP

Antigène: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Autre désignation: MLF1IP (MLF1IP Produits)

Synonymes: anticorps MGC147270, anticorps CENP50, anticorps CENPU, anticorps CENPU50, anticorps KLIP1, anticorps PBIP1, anticorps CENP-U, anticorps CENP-50, anticorps 1700029A22Rik, anticorps centromere protein U, anticorps MLF1 interacting protein, anticorps cenpu, anticorps MLF1IP, anticorps CENPU, anticorps Cenpu

Sujet: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

ID gène: 79682

UniProt: Q71F23