CRYAB anticorps (FITC)

N° du produit ABIN2481828

Cet anticorps anti-CRYAB est un anticorps Souris Monoclonal détectant CRYAB dans WB, ELISA, IHC, IF et ICC. Adapté pour Humain.

Aperçu rapide pour CRYAB anticorps (FITC) (ABIN2481828)

Antigène: CRYAB (Crystallin, alpha B (CRYAB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CRYAB est conjugé à/à la FITC

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3A10-C9

Détail du produit anti-CRYAB anticorps (FITC)

Specificité: Detects ~20 kDa (predicted mol.weight is ~21 kDa). Does not cross-react with αA-crystallin, βL-crystallin, ΒH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Réactivité croisée: Boeuf (Vache), Humain, Rat

Purification: Protein G Purified

Immunogène: Native Alpha B Crystallin

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2481828 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur CRYAB

Antigène: CRYAB (Crystallin, alpha B (CRYAB))

Autre désignation: Alpha B Crystallin

Sujet: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

ID gène: 1410

NCBI Accession: NP_001876

UniProt: P02511