LAMP2 anticorps (HRP)

N° du produit ABIN2484612

Cet anticorps Rat Monoclonal détecte spécifiquement LAMP2 dans WB, IP, ICC et IF. Il présente une réactivité envers Souris.

Aperçu rapide pour LAMP2 anticorps (HRP) (ABIN2484612)

Antigène: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP2 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GL2A7

Détail du produit anti-LAMP2 anticorps (HRP)

Specificité: Detects ~100-110 kDa.

Réactivité croisée: Humain, Souris, Lapin

Purification: Protein G Purified

Immunogène: Purified preparation of mouse liver lysosomal membranes

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:500)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2484612 was sufficient for detection of LAMP2 in 20 μg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur LAMP2

Antigène: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Autre désignation: LAMP2

Sujet: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

ID gène: 16784

NCBI Accession: NP_001017959

UniProt: P17047

Pathways: Autophagy