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HSF1 anticorps (AA 378-395) (Biotin)

HSF1 Reactivité: Souris WB, ELISA, IHC, IP, IF, GS, ICC Hôte: Rat Monoclonal 10H8 Biotin
N° du produit ABIN2484627
  • Antigène Voir toutes HSF1 Anticorps
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Épitope
    • 29
    • 15
    • 12
    • 12
    • 12
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 378-395
    Reactivité
    • 193
    • 108
    • 57
    • 13
    • 11
    • 10
    • 7
    • 7
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 179
    • 32
    • 7
    • 1
    Rat
    Clonalité
    • 168
    • 50
    Monoclonal
    Conjugué
    • 118
    • 12
    • 11
    • 10
    • 8
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp HSF1 est conjugé à/à la Biotin
    Application
    • 182
    • 110
    • 84
    • 65
    • 47
    • 43
    • 30
    • 28
    • 28
    • 27
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
    Specificité
    Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).
     Réactivité croisée
    Boeuf (Vache), Cobaye, Hamster, Humain, Singe, Souris, Lapin, Rat
    Purification
    Protein G Purified
    Immunogène
    Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
    Clone
    10H8
    Isotype
    IgG1
    Top Product
    Discover our top product HSF1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:1000)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2484627 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Autre désignation
    HSF1 (HSF1 Produits)
    Synonymes
    anticorps hsf1, anticorps Hsf, anticorps xhsf1, anticorps AA960185, anticorps HSTF1, anticorps HSF1, anticorps HSF, anticorps HSF1c, anticorps hsf1a, anticorps hsf1b, anticorps zHSF1a, anticorps zHSF1b, anticorps hstf1, anticorps heat shock factor, anticorps Heat Shock Factor, anticorps heat shock factor 1, anticorps heat shock transcription factor 1, anticorps heat shock transcription factor 1 S homeolog, anticorps hsf1, anticorps hsf-1, anticorps Hsf1, anticorps HSF1, anticorps hsf1.S
    Sujet
    HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
    ID gène
    15499
    NCBI Accession
    NP_032322
    UniProt
    P38532
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