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HDEL anticorps (C-Term) (Atto 488)

HDEL Reactivité: Saccharomyces cerevisiae WB, IF, ICC Hôte: Souris Monoclonal 2E7 Atto 488
N° du produit ABIN2484704
  • Antigène Tous les produits HDEL
    HDEL
    Épitope
    C-Term
    Reactivité
    • 22
    • 12
    • 11
    • 10
    • 10
    • 10
    • 10
    • 10
    • 1
    Saccharomyces cerevisiae
    Hôte
    • 22
    Souris
    Clonalité
    • 22
    Monoclonal
    Conjugué
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp HDEL est conjugé à/à la Atto 488
    Application
    • 22
    • 12
    • 10
    • 10
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~78 kDa.
     Réactivité croisée
    Drosophila melanogaster, Plantes, Saccharomyces cerevisiae
    Purification
    Protein G Purified
    Immunogène
    Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
    Clone
    2E7
    Isotype
    IgG2b
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2484704 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    HDEL
    Autre désignation
    HDEL (HDEL Produits)
    Sujet
    HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
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